Sterility:
Article Title: Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation.
Article Snippet: .. Table-top centrifuge (refrigerated; e.g., Thermo Fisher #75002471) Sterile, RNAse-free Eppendorf tubes Sterile, RNAse-free pipette tips Depending on the expected RNA quantity: Qubit and necessary reagents (<40 ng/μl; e.g., Thermo Fisher #Q33217) or Nanodrop Spectrophotometer (> 40 ng/μl; e.g., Thermo Fisher #ND-2000) RNA integrity control Agilent BioAnalyzer (Agilent #G2939BA) or TapeStation (Agilent #G2991AA; RRID:SCR_014994) In case of low quantities: BioAnalzyer Pico Kit (< 5 ng/μl) (Agilent #5067-1513) or TapeStation High Sensitivity RNA Screentape and sample buffer (<10 ng/μl) (Agilent #5067-5581 and #5067-5580) In case of medium to high quantities (> 25 ng/μl): BioAnalyzer Nano Kit (Agilent #5067-1511) or TapeStation RNA Screentape and sample buffer (Agilent #5067- 5576 and #5067-5577) Preparation of samples Although tissues obtained using the described technique can be potentially used for any -omics profiling, transcriptomic and proteomic screening are the most commonly used approaches. ..
Article Title: Isolation of Neutrophil Nuclei for Use in NETosis Assays
Article Snippet: .. Pipette tips (1000 μl, 200 μl, 10 μl) Tissue, Kimwipes disposable wipers (Sigma-Aldrich, catalog number: Z188956-1PAK) 6-well plate, sterile tissue culture grade (Sigma-Aldrich, Corning, catalog number: SIAL0506-100EA) 15 ml sterile conical tubes (Sigma-Aldrich, Greiner, catalog number: T1943-1000EA) 1.5 ml tubes sterile (Eppendorf, catalog number: 0030 120.086) 27 G needle (Sigma-Aldrich, catalog number: Z192384-100EA) 10 ml disposable plastic syringe (Fischer Scientific, catalog number: 15889152) 12 weeks-old mouse C57BL/6J EasySep neutrophil enrichment kit (StemCell Technology, catalog number: 19762) Ethanol RPMI 1640 (Thermo Fisher Scientific, Gibco, catalog number: 61870010) TC grade 1 M HEPES (Thermo Fisher Scientific, Gibco, catalog number: 15630080) Trypan blue solution (Thermo Fisher Scientific, catalog number: 15250061) ATP sodium (Sigma-Aldrich, catalog number: A7699-1G) Magnesium Chloride (MgCl 2 ) (Sigma-Aldrich, catalog number: M2670) Potassium Chloride (KCl) (Sigma-Aldrich, catalog number: P9333) Phenylmethanesulfonyl fluoride (PMSF) (Sigma-Aldrich, Roche, catalog number: PMSF-RO) cOmplete Mini, EDTA-free protease inhibitor tablets (Sigma-Aldrich, Roche, catalog number: 11836170001) Opti-MEM, reduced-serum media (Thermo Fisher Scientific, catalog number: 31985062) 70% Ethanol solution (see Recipes) Neutrophil media (see Recipes) PMSF stock solution (see Recipes) ATP stock solution (see Recipes) Hypotonic buffer (see Recipes) .. P1000 micropipette Scissors Hematocytometer Centrifuge (Eppendorf, model: 5415C) Incubator -80 °C freezer Cell disruption vessel, 45 ml (Parr Instrument Company, catalog number: 4639) Nitrogen tank linked to a pressure-reducing valve Mouse Dissecting kit (World Precision Instrument) Tissue culture hood
Article Title: Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen.
Article Snippet: The glomerulonephritides are diseases characterized by immune-mediated glomerular inflammation.. Most severe and rapidly progressive forms of glomerulonephritis feature the participation of injurious leukocytes that localize to glomeruli.. This unit describes classical models of rapidly progressive glomerulonephritis in mice, induced by injecting heterologous globulin (raised in sheep) that binds to the glomerular basement membrane.
Transferring:Article Title: Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation.
Article Snippet: .. Table-top centrifuge (refrigerated; e.g., Thermo Fisher #75002471) Sterile, RNAse-free Eppendorf tubes Sterile, RNAse-free pipette tips Depending on the expected RNA quantity: Qubit and necessary reagents (<40 ng/μl; e.g., Thermo Fisher #Q33217) or Nanodrop Spectrophotometer (> 40 ng/μl; e.g., Thermo Fisher #ND-2000) RNA integrity control Agilent BioAnalyzer (Agilent #G2939BA) or TapeStation (Agilent #G2991AA; RRID:SCR_014994) In case of low quantities: BioAnalzyer Pico Kit (< 5 ng/μl) (Agilent #5067-1513) or TapeStation High Sensitivity RNA Screentape and sample buffer (<10 ng/μl) (Agilent #5067-5581 and #5067-5580) In case of medium to high quantities (> 25 ng/μl): BioAnalyzer Nano Kit (Agilent #5067-1511) or TapeStation RNA Screentape and sample buffer (Agilent #5067- 5576 and #5067-5577) Preparation of samples Although tissues obtained using the described technique can be potentially used for any -omics profiling, transcriptomic and proteomic screening are the most commonly used approaches. ..
Article Title: Isolation of Neutrophil Nuclei for Use in NETosis Assays
Article Snippet: .. Pipette tips (1000 μl, 200 μl, 10 μl) Tissue, Kimwipes disposable wipers (Sigma-Aldrich, catalog number: Z188956-1PAK) 6-well plate, sterile tissue culture grade (Sigma-Aldrich, Corning, catalog number: SIAL0506-100EA) 15 ml sterile conical tubes (Sigma-Aldrich, Greiner, catalog number: T1943-1000EA) 1.5 ml tubes sterile (Eppendorf, catalog number: 0030 120.086) 27 G needle (Sigma-Aldrich, catalog number: Z192384-100EA) 10 ml disposable plastic syringe (Fischer Scientific, catalog number: 15889152) 12 weeks-old mouse C57BL/6J EasySep neutrophil enrichment kit (StemCell Technology, catalog number: 19762) Ethanol RPMI 1640 (Thermo Fisher Scientific, Gibco, catalog number: 61870010) TC grade 1 M HEPES (Thermo Fisher Scientific, Gibco, catalog number: 15630080) Trypan blue solution (Thermo Fisher Scientific, catalog number: 15250061) ATP sodium (Sigma-Aldrich, catalog number: A7699-1G) Magnesium Chloride (MgCl 2 ) (Sigma-Aldrich, catalog number: M2670) Potassium Chloride (KCl) (Sigma-Aldrich, catalog number: P9333) Phenylmethanesulfonyl fluoride (PMSF) (Sigma-Aldrich, Roche, catalog number: PMSF-RO) cOmplete Mini, EDTA-free protease inhibitor tablets (Sigma-Aldrich, Roche, catalog number: 11836170001) Opti-MEM, reduced-serum media (Thermo Fisher Scientific, catalog number: 31985062) 70% Ethanol solution (see Recipes) Neutrophil media (see Recipes) PMSF stock solution (see Recipes) ATP stock solution (see Recipes) Hypotonic buffer (see Recipes) .. P1000 micropipette Scissors Hematocytometer Centrifuge (Eppendorf, model: 5415C) Incubator -80 °C freezer Cell disruption vessel, 45 ml (Parr Instrument Company, catalog number: 4639) Nitrogen tank linked to a pressure-reducing valve Mouse Dissecting kit (World Precision Instrument) Tissue culture hood
Spectrophotometry:Article Title: Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation.
Article Snippet: .. Table-top centrifuge (refrigerated; e.g., Thermo Fisher #75002471) Sterile, RNAse-free Eppendorf tubes Sterile, RNAse-free pipette tips Depending on the expected RNA quantity: Qubit and necessary reagents (<40 ng/μl; e.g., Thermo Fisher #Q33217) or Nanodrop Spectrophotometer (> 40 ng/μl; e.g., Thermo Fisher #ND-2000) RNA integrity control Agilent BioAnalyzer (Agilent #G2939BA) or TapeStation (Agilent #G2991AA; RRID:SCR_014994) In case of low quantities: BioAnalzyer Pico Kit (< 5 ng/μl) (Agilent #5067-1513) or TapeStation High Sensitivity RNA Screentape and sample buffer (<10 ng/μl) (Agilent #5067-5581 and #5067-5580) In case of medium to high quantities (> 25 ng/μl): BioAnalyzer Nano Kit (Agilent #5067-1511) or TapeStation RNA Screentape and sample buffer (Agilent #5067- 5576 and #5067-5577) Preparation of samples Although tissues obtained using the described technique can be potentially used for any -omics profiling, transcriptomic and proteomic screening are the most commonly used approaches. ..
Control:Article Title: Neocortical Microdissection at Columnar and Laminar Resolution for Molecular Interrogation.
Article Snippet: .. Table-top centrifuge (refrigerated; e.g., Thermo Fisher #75002471) Sterile, RNAse-free Eppendorf tubes Sterile, RNAse-free pipette tips Depending on the expected RNA quantity: Qubit and necessary reagents (<40 ng/μl; e.g., Thermo Fisher #Q33217) or Nanodrop Spectrophotometer (> 40 ng/μl; e.g., Thermo Fisher #ND-2000) RNA integrity control Agilent BioAnalyzer (Agilent #G2939BA) or TapeStation (Agilent #G2991AA; RRID:SCR_014994) In case of low quantities: BioAnalzyer Pico Kit (< 5 ng/μl) (Agilent #5067-1513) or TapeStation High Sensitivity RNA Screentape and sample buffer (<10 ng/μl) (Agilent #5067-5581 and #5067-5580) In case of medium to high quantities (> 25 ng/μl): BioAnalyzer Nano Kit (Agilent #5067-1511) or TapeStation RNA Screentape and sample buffer (Agilent #5067- 5576 and #5067-5577) Preparation of samples Although tissues obtained using the described technique can be potentially used for any -omics profiling, transcriptomic and proteomic screening are the most commonly used approaches. ..
Protease Inhibitor:Article Title: Isolation of Neutrophil Nuclei for Use in NETosis Assays
Article Snippet: .. Pipette tips (1000 μl, 200 μl, 10 μl) Tissue, Kimwipes disposable wipers (Sigma-Aldrich, catalog number: Z188956-1PAK) 6-well plate, sterile tissue culture grade (Sigma-Aldrich, Corning, catalog number: SIAL0506-100EA) 15 ml sterile conical tubes (Sigma-Aldrich, Greiner, catalog number: T1943-1000EA) 1.5 ml tubes sterile (Eppendorf, catalog number: 0030 120.086) 27 G needle (Sigma-Aldrich, catalog number: Z192384-100EA) 10 ml disposable plastic syringe (Fischer Scientific, catalog number: 15889152) 12 weeks-old mouse C57BL/6J EasySep neutrophil enrichment kit (StemCell Technology, catalog number: 19762) Ethanol RPMI 1640 (Thermo Fisher Scientific, Gibco, catalog number: 61870010) TC grade 1 M HEPES (Thermo Fisher Scientific, Gibco, catalog number: 15630080) Trypan blue solution (Thermo Fisher Scientific, catalog number: 15250061) ATP sodium (Sigma-Aldrich, catalog number: A7699-1G) Magnesium Chloride (MgCl 2 ) (Sigma-Aldrich, catalog number: M2670) Potassium Chloride (KCl) (Sigma-Aldrich, catalog number: P9333) Phenylmethanesulfonyl fluoride (PMSF) (Sigma-Aldrich, Roche, catalog number: PMSF-RO) cOmplete Mini, EDTA-free protease inhibitor tablets (Sigma-Aldrich, Roche, catalog number: 11836170001) Opti-MEM, reduced-serum media (Thermo Fisher Scientific, catalog number: 31985062) 70% Ethanol solution (see Recipes) Neutrophil media (see Recipes) PMSF stock solution (see Recipes) ATP stock solution (see Recipes) Hypotonic buffer (see Recipes) .. P1000 micropipette Scissors Hematocytometer Centrifuge (Eppendorf, model: 5415C) Incubator -80 °C freezer Cell disruption vessel, 45 ml (Parr Instrument Company, catalog number: 4639) Nitrogen tank linked to a pressure-reducing valve Mouse Dissecting kit (World Precision Instrument) Tissue culture hood
Plasmid Purification:Article Title: Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen.
Article Snippet: The glomerulonephritides are diseases characterized by immune-mediated glomerular inflammation.. Most severe and rapidly progressive forms of glomerulonephritis feature the participation of injurious leukocytes that localize to glomeruli.. This unit describes classical models of rapidly progressive glomerulonephritis in mice, induced by injecting heterologous globulin (raised in sheep) that binds to the glomerular basement membrane.
Injection:Article Title: Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen.
Article Snippet: The glomerulonephritides are diseases characterized by immune-mediated glomerular inflammation.. Most severe and rapidly progressive forms of glomerulonephritis feature the participation of injurious leukocytes that localize to glomeruli.. This unit describes classical models of rapidly progressive glomerulonephritis in mice, induced by injecting heterologous globulin (raised in sheep) that binds to the glomerular basement membrane.
Immunohistochemistry:Article Title: Glomerulonephritis Induced by Heterologous Anti-GBM Globulin as a Planted Foreign Antigen.
Article Snippet: The glomerulonephritides are diseases characterized by immune-mediated glomerular inflammation.. Most severe and rapidly progressive forms of glomerulonephritis feature the participation of injurious leukocytes that localize to glomeruli.. This unit describes classical models of rapidly progressive glomerulonephritis in mice, induced by injecting heterologous globulin (raised in sheep) that binds to the glomerular basement membrane.
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